Neuron-specific splicing regulation analysis through full-length transcriptomic approaches

Universite de Bordeaux, France

Cell differentiation is the product of tightly regulated gene expression programs. The nematode C. elegans, being the only multicellular organism with a fully characterized cell lineage , is the ideal model to study the molecular principles that define cellular identity. Recent work from the C. elegans Neuronal Gene Expression Map & Network (CeNGEN) Project demonstrated the feasibility of systematically characterizing the mRNA content of individual neurons in C. elegans. While the scRNA-seq data is a tremendous resource to understand transcriptional regulatory networks, the authors noted that the identification of alternatively spliced isoforms could only be achieved through bulk sequencing of isolated neuron types.

Research team webpage: https://arna.cnrs.fr/Dupuy_Lab/Welcome.html